Application Pool will be found in IIS Manager:
Unless you added some pools like I did, there should be one there called DefaultAppPool. Select it, right click and then select the Identity Tab. Ensure that pre-defined is selected and that it shows Network Service as the Account to use for that pool.
The next step is to check the file permissions of your web site. You need to navigate to the root folder of your web site, for example: c:\inetpub\wwwroot\. Select the folder, right click the folder and select Properties, then select the Security Tab.
You want to ensure that the wwwroot folder has at a minimum these permissions:
These services should be there:
Network Service
Interactive
System (all the above should have Full Control)
Network (List Folders only).

If you do not see this type of Tab, What Operating System are you using and what file system do you have installed (FAT, FAT32, NTFS)?
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